Sequence and analysis of the DNA encoding protective antigen of Bacillus anthracis
作者： S.L. Welkos , J.R. Lowe , F. Eden-McCutchan , M. Vodkin , S.H. Leppla
DOI: 10.1016/0378-1119(88)90439-8
关键词: Molecular biology 、 Peptide sequence 、 Open reading frame 、 Genetics 、 Start codon 、 Nucleic acid sequence 、 Gene 、 Biology 、 Signal peptide 、 Stop codon 、 Codon usage bias
摘要: Abstract The nucleotide sequence of the protective antigen (PA) gene from Bacillus anthracis and 5′ 3' flanking sequences were determined. PA is one three proteins comprising anthrax toxin; its first to be reported B. . open reading frame (ORF) 2319 bp long, which 2205 encode 735 amino acids secreted protein. This region preceded by 29 codons, appear a signal peptide having characteristics in common with those other proteins. A consensus TATAAT was located at putative −10 promoter site. Shine-Dalgarno site similar that found genes sp. 7 upstream ATG start codon. codon usage for reflected high + T (69%) base composition differed bacterial most examined. TAA translation stop followed an inverted repeat forming potential termination signal. In addition, 192-codon ORF unknown significance, theoretically encoding 21.6-kDa protein, end gene.
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