The amino acid sequence of a crystal protein from Bacillus thuringiensis deduced from the DNA base sequence.
作者： H E Schnepf , H C Wong , H R Whiteley
DOI: 10.1016/S0021-9258(18)88966-9
关键词:
摘要: We have determined the nucleotide sequence of a 4222-base segment DNA which contains promoter, coding region, and terminator crystal protein gene cloned from Bacillus thuringiensis plasmid. A 1176 amino acids encoding Mr 133,500 peptide was deduced single open reading frame. This protein-coding region analyzed for codon usage, predicted hydropathy, secondary structure. Examination base revealed presence several inverted direct repeats located in both noncoding regions. S1 nuclease mapping used to locate transcription termination point at site following potentially very stable stem-and-loop
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