Hydroxyl radical attack on dopamine.
作者： A Slivka , G Cohen
DOI: 10.1016/S0021-9258(17)36277-4
关键词: Organic chemistry 、 Ferrous 、 Hydrogen peroxide 、 Dimethyl sulfoxide 、 Medicinal chemistry 、 Hydroxylation 、 Chemistry 、 Redox 、 Hydroxyl radical 、 Ethanol 、 Radical
摘要: Hydroxyl radicals were generated in the presence of 1 mM dopamine (3,4-dihydroxyphenylethylamine) at pH 7.2 (50 phosphate buffer) by following two mechanisms: 1) a classic Fenton-type reaction between hydrogen peroxide and ferrous chelate (ferrous diethylenetriaminepentaacetate) 2) cyclical redox reactions iron-EDTA/ascorbate. Three ring-monohydroxylated products detected high performance liquid chromatography with electrochemical detection: 2-hydroxydopamine, 5-hydroxydopamine, 6-hydroxydopamine an approximate ratio 3:2:1. Scavengers hydroxyl (dimethyl sulfoxide, mannitol, ethanol) suppressed yields concentration-dependent manner. The formation nonphysiologic hydroxylated forms can provide probe for neurons.
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