Total Synthesis of Acetate from CO2 II. Purification and Properties of Formyltetrahydrofolate Synthetase from Clostridium thermoaceticum

作者： Albert Y. Sun , Lars Ljungdahl , Harland G. Wood

DOI: 10.1128/JB.98.2.842-844.1969

关键词: Biology 、 Formate 、 Divalent metal 、 Formyltetrahydrofolate synthetase 、 Kinetics 、 Total synthesis 、 Clostridium 、 Biochemistry 、 Clostridium thermoaceticum 、 Adenosine triphosphate 、 Molecular biology 、 Microbiology

摘要: Formyltetrahydrofolate synthetase from Clostridium thermoaceticum had optimal activity at 55 to 60 C. K(m) values were: adenosine triphosphate (ATP), 6.25 x 10(-4)m; formate, 1.67 10(-3)m; and tetrahydrofolate, 1.00 pH 8.0. There was a requirement for divalent metals specifically ATP.