Assembly of the tendon extracellular matrix during development.

摘要: The assembly of the collagenous extracellular matrix during tendon development was studied to determine mechanisms involved in collagen fibril growth. Developing avian metatarsal tendons were using structural, immunochemical and biochemical approaches. Tendon fibroblasts shown establish a hierarchy compartments associated with fibrils, bundles, macroaggregates development. These distinct domains provide mechanism for fibroblast influence steps assembly. A discontinuous fibrillar assembled segments approximately 10-20 microns long deposited into bundles by 14 d embryo fibroblasts. segment is normal intermediate, permitting orderly linear, lateral intercalatory and/or linear fusion may be responsible formation mature continuous fibrils. Fibril isolated from 12-18 chick tendons. Homogenisation almost completely disrupted 12-15 Transmission electron microscopy demonstrated intact segments. Between 12 15 development, mean length increased 22 33 microns. incremental increase indicates limited which supported morphological examination. At 16 d, there significant decrease extractability 17-18 unextractable. Mean lengths 37 > 70 17 respectively. During this period, diameter also increased.(ABSTRACT TRUNCATED AT 250 WORDS)