Suitability of Nanoparticles to Face Benzo(a)pyrene-Induced Genetic and Chromosomal Damage in M. galloprovincialis. An In Vitro Approach.

摘要: Benzo(a)pyrene (B(a)P) is a well-known genotoxic agent, the removal of which from environmental matrices mandatory, necessitating application cleaning strategies that are harmless to human and health. The potential nanoparticles (NPs) in remediation polluted environments increasing interest. Here, specifically designed NPs were selected as being non-genotoxic able interact with B(a)P, order address genetic chromosomal damage it produces. A newly formulated pure anatase nano-titanium (nano-TiO2), commercial mixture rutile anatase, carbon black-derived hydrophilic (HNP) applied. Once had been ascertained for work did not induce genotoxicity, marine mussel gill biopsies exposed vitro B(a)P (2 μg/mL), alone combination (50 µg/mL nano-TiO2, 10 HNP). DNA primary reversible was evaluated by means Comet assay. Chromosomal persistent assessed on basis micronuclei frequency nuclear abnormalities Micronucleus-Cytome Transmission Electron Microscopy (TEM) performed investigate mechanism action exerted NPs. Pure Anatase n-TiO2 found be most suitable our purpose, cyto- genotoxicity free reduce associated exposure B(a)P.