Interleukin‐6 and interleukin‐10 are expressed in organs of normal young and old mice

摘要: Interleukin-6 (IL-6) is a pleiotropic inflammatory cytokine, also endowed with inflammation-inhibiting properties. The status of interleukin-10 (IL-10) as an anti-inflammatory cytokine more solidly established. roles IL-6 and IL-10 in the context organ physiology, their possible modulation by aging process, are not satisfactorily understood. purpose this work was to characterize expression different cellular compartments mice, under steady-state stress conditions. former evaluated immunohistochemistry (IHC) enzyme-linked immunosorbent assay (ELISA) analyses lysates (LYS) (addressing intracellular compartment), while latter assessed ELISA organ-conditioned media (CM), obtained after 48 hrs culturing potential secretion/diffusion). Under conditions, overall level relatively low both age groups (exceptionally, IHC staining demonstrated enhanced these cytokines heart, skeletal muscle brain young mice). Much elevated levels were CM, possibly emphasizing role stress. This most characteristically shown highly specialized organs (heart, kidney) liver old compared other lymphoreticular (lungs, spleen, small intestine) tested. Thus, markedly upregulated organs, considerably reduced organs. In addition, appears be associated altered patterns secretion/diffusion potentials heart muscle, on one hand, increased detection other. These findings may contribute better understanding unique functions various groups, suggest important response age.