The mink gene for the λ light immunoglobulin chain: Characterization of cDNA and chromosomal localization
作者： T. M. Khlebodarova , N. M. Matveeva , O. L. Serov , A. M. Najakshin , E. S. Belousov
DOI: 10.1007/BF00353856
关键词: Immunoglobulin gene 、 Coding region 、 Molecular biology 、 Genetics 、 Biology 、 Homology (biology) 、 Complementary DNA 、 Immunoglobulin light chain 、 Mink 、 cDNA library 、 Gene
摘要: A cDNA library from mink spleen was constructed by use of the phage λgt11. The screened using polyvalent serum raised against immunoglobulin λ chain. As a result, several clones expressing light chains were identified. Sequencing one with an 803 bp insert performed. comprised nearly entire coding region for mature gene exception leader polypeptide and amino acids RF1 V segment. Compared rabbit, mouse human genes, homology cloned sequence found to be highest relative rabbit gene. With containing C-region only as probe, DNAs mink-Chinese hamster hybrid studied. results segregation analysis this chromosomes in clone panel allowed us assign constant (IGLC) Chromosome (Chr) 4.
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