Combined effect of nisin and pulsed electric fields on the inactivation of Escherichia coli.
作者： MAURICIO R. TEREBIZNIK , ROSA J. JAGUS , PATRICIA CERRUTTI , MARTA S. DE HUERGO , ANA M. R. PILOSOF
DOI: 10.4315/0362-028X-63.6.741
关键词: Bacteriocin 、 Microbiology 、 Food preservation 、 Bacteria 、 Enterobacteriaceae 、 Food science 、 Ultrafiltration (renal) 、 Biology 、 Escherichia coli 、 Nisin 、 Action spectrum
摘要: The Doehlert design was applied in order to investigate the combined effect of nisin and high voltage pulsed electric fields (PEF) on inactivation Escherichia coli simulated milk ultrafiltrate media. Nisin alone totally inactivated by PEF, but presence bacterial cells a protective observed. However, effectiveness still decreased when were subjected treatment. In spite this phenomenon, an almost additive response emerged as consequence A 4-log cycle reduction may be accomplished with around 1,000 IU/ml (7.15 μM) three pulses 11.25 kV/cm or 500 for five same intensity. observed efficacy arising from combination both treatments suggests possibility using PEF improving action spectrum natural antimicrobials.
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