A novel membrane overlay cultivation technique for the measurement of growth and production of mycotoxins by Penicillium commune and Aspergillus flavus
作者： Nceba Gqaleni , JohnE. Smith , John Lacey
DOI: 10.1007/BF00222566
关键词: Aspergillus 、 Mycotoxin 、 Agar 、 Microbiology 、 Agar plate 、 Aspergillus flavus 、 Mycelium 、 Penicillium commune 、 Cyclopiazonic acid 、 Food science 、 Biology
摘要: Penicillium commune and Aspergillus flavus were grown on a microporous membrane (pore diameter 2.4 nm) overlaying an agar medium. The mycelium was easily separated from the disc assayed for dry weight, protein DNA content, while concentrations of total aflatoxins cyclopiazonic acid determined in underlying agar. Mycotoxin production commenced during exponential phase growth both toxins still increasing after 7 days incubation. Toxins detected 1–2 before they could be
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