Tritium (3H) radiolabeling of protein A and antibody to high specific activity: application to cell surface antigen radioimmunoassays.
作者： Ronald L. Wilder , Catherine C. Yuen , Bondada Subbarao , Virgil L. Woods , Cornelius B. Alexander
DOI: 10.1016/0022-1759(79)90192-3
关键词: Antibody 、 Protein A 、 Biochemistry 、 Antigen 、 Bioassay 、 Chemistry 、 Radioimmunoassay 、 Cell 、 Sodium borohydride 、 Tritium
摘要: Abstract Staphylococcal protein A and several different immunoglobulins have been radiolabeled to high specific activities (>106 cpm/μg) by reductive methylation with tritiated (3H) sodium borohydride. The proteins retain excellent functional antigenic properties. utility of these reagents in a variety assays for cell surface antigens is illustrated. results indicate that this radiolabeling procedure may become the method choice many solution immunoassays.
elsevier.com
sci-hub.se 参考文章(12)
Mildred Cohn, George R. Drysdale, A STUDY WITH O18 OF ADENOSINE TRIPHOSPHATE FORMATION IN OXIDATIVE PHOSPHORYLATION Journal of Biological Chemistry. ,vol. 216, pp. 831- 846 ,(1955) , 10.1016/S0021-9258(19)81437-0
A. E. Bolton, W. M. Hunter, The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Application to the radioimmunoassay Biochemical Journal. ,vol. 133, pp. 529- 538 ,(1973) , 10.1042/BJ1330529
Aftab A. Ansari, Rose G. Mage, Immunochemical studies of the a allotypes of rabbit heavy chain variable regions—I. Immunochemistry. ,vol. 15, pp. 561- 568 ,(1978) , 10.1016/0161-5890(78)90009-3
Ronald L. Wilder, Catherine Chew Yuen, Rose G. Mage, Lactoperoxidase Catalyzed Radioiodination of Cell Surface Immunoglobulin: Incorporated Radioactivity May Not Reflect Relative Cell Surface Ig Density Journal of Immunology. ,vol. 122, pp. 459- 463 ,(1979)
P. Parham, C. Terhorst, H. Herrmann, R. E. Humphreys, M. D. Waterfield, J. L. Strominger, Immunological and chemical purity of papain-solubilized HL-A antigens. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 72, pp. 1594- 1598 ,(1975) , 10.1073/PNAS.72.4.1594
Theodore Werblin, Rose Mage, A screening assay for rapid and quantitative measurement of immunoglobulins and anti-immunoglobulins in unknown sera. Journal of Immunological Methods. ,vol. 16, pp. 337- 350 ,(1977) , 10.1016/S0022-1759(97)90004-1
Gary E. Means, Robert E. Feeney, Reductive alkylation of amino groups in proteins. Biochemistry. ,vol. 7, pp. 2192- 2201 ,(1968) , 10.1021/BI00846A023
W. M. HUNTER, F. C. GREENWOOD, Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific Activity Nature. ,vol. 194, pp. 495- 496 ,(1962) , 10.1038/194495A0
G. Dorval, K.I. Welsh, H. Wigzell, A radioimmunoassay of cellular surface antigens on living cells using iodinated soluble protein a from Staphylococcus aureus Journal of Immunological Methods. ,vol. 7, pp. 237- 249 ,(1975) , 10.1016/0022-1759(75)90021-6
Martin Morrison, Gladys S. Bayse, Catalysis of iodination by lactoperoxidase. Biochemistry. ,vol. 9, pp. 2995- 3000 ,(1970) , 10.1021/BI00817A010