Agrobacterium rhizogenes-mediated transformation of Rubia peregrina L.: in vitro accumulation of anthraquinones
作者： Azhar H. Lodhi , Barry V. Charlwood
DOI: 10.1007/BF00034842
关键词: Kanamycin 、 Transformation (genetics) 、 Callus 、 Alizarin 、 Rubia peregrina 、 Rhizobiaceae 、 Biology 、 Anthraquinones 、 Agrobacterium 、 Botany
摘要: An Agrobacterium rhizogenes-mediated transformation system for Rubia peregrina L. has been established by co-cultivation of callus cultures or direct infection explants with A. rhizogenes LBA 9402 harbouring the binary vector pMON 9703 containing gus and npt-II genes as markers. The putative transformed roots were selected on medium kanamycin (25 mg l-1). Antibiotic resistant root clones subjected to histochemical analysis localisation β-glucuronidase activity. Polymerase chain reaction was used confirm presence gus, T L border sequences in clones. Spontaneous regeneration shoots observed from 30 day-old transgenic roots. Total anthraquinone alizarin contents measured spectrophotometry high performance liquid chromatography. accumulation total anthraquinones found be approximately 2-fold higher than that one year-old field grown (2.12±0.12 1.23±0.12 g-1 dry weight, respectively). Alizarin major 3-fold
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