Methyl jasmonate increases the production of valepotriates by transformed root cultures of Valerianella locusta

摘要: Transformed roots of V. locusta (Valerianaceae) were obtained through transformation with Agrobacterium rhizogenes strains A4 and ATCC 15834. Six known valepotriates, including diavaltrate, acevaltrate, didrovaltrate, IVHD-valtrate, isovaltrate, valtrate the major components detected. An LC/PDA method was used in quantitation these compounds transformed root extracts. The treatment biotic (methyl jasmonate, salicylic acid, yeast extract) abiotic elicitors (CuSO4, HgCl2, CaCl2) as a strategy to improve production valepotriates. Methyl jasmonate appeared be best elicitor for valepotriate production, yielding up 7-fold increase total content, while HgCl2 had most deteriorating effect on Salicylic acid-, CuSO4- CaCl2-treated showed significant increases at short duration exposure; decreased time elicitation increased. highest content achieved this study 139 mg g−1 DW (13.9%) from treated 10 days 100 μM methyl jasmonate. This amount >50- 12-fold higher than values reported cultivated plants callus culture, respectively, comparable high valepotriate-producing species Valeriana thalictroides Graebn. isovaltrate significantly higher, IVHD-valtrate lower that similar control. IVAL/VAL ratio affected by but not other elicitors. use cultures combination offer new route production.