Enhanced green fluorescence protein/layered double hydroxide composite ultrathin films: bio-hybrid assembly and potential application as a fluorescent biosensor

作者: Ping Zhang , Yuehua Hu , Ruili Ma , Ling Li , Jun Lu

DOI: 10.1039/C6TB02638A

关键词: StackingMoleculeNanosheetFluorescenceDrug deliveryBiophysicsMaterials scienceGreen fluorescent proteinNanotechnologyHydroxideBiosensor

摘要: Protein immobilization is of significant interest for applications in biosensing, drug delivery and bioconversion, and challenges still remain for the in vitro immobilization and application of proteins. Due to it being non-specific to species, easy to express in cells and able to exhibit fluorescence after expression without the need for cofactors or chaperones, green fluorescent protein (GFP), together with its differently colored mutants, has been widely studied and applied. This article reports the fabrication of enhanced green fluorescent …

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