Onset of Accumulation of PrPresin Murine ME7 Scrapie in Relation to Pathological and PrP Immunohistochemical Changes

摘要: Abstract In a murine scrapie model, three different methods (immunohistochemistry, Western blotting and histoIblotting) for determining disease-specific PrP accumulation were compared. The incubation period of ME7 in the F1 cross C57 BL VM/Dk mice is about 230 days. Mice show hippocampal neuronal loss from 160–180 days post-inoculation (dpi), CA1 neuron dendritic spine atrophy at 126 dpi, axon terminal degeneration synaptic 84–98 dpi. Infectivity titres least 100 are present 40 was detected immunohistochemically 60 dpi hippocampus thalamus. Thus, precedes even earliest neurodegenerative changes. Low amounts immunolabelling found between after which intensity increased markedly. histoblot method PrPresin one four whole brains first identified PrPresat 80 our hands, most sensitive detecting accumulations immunohistochemical examination. However, unable to distinguish normal abnormal isoforms PrP. It therefore possible that initial takes place as PrPsenand translation PrPsento PrPresdoes not take until later stages disease process. lags behind development infectivity titres. relative rates increase titre different, suggesting these parameters may be measures biological events.