Stability of murine scrapie strain 87V after passage in sheep and comparison with the CH1641 ovine strain.

摘要: Breed- and prion protein (PRNP) genotype-related disease phenotype variability has been observed in sheep infected with the 87V murine scrapie strain. Therefore, stability of this strain was tested by inoculating sheep-derived brain material back into VM mice. As some sheep-adapted phenotypes were reminiscent CH1641 scrapie, transgenic mice (Tg338) expressing ovine (PrP) inoculated same sources CH1641. Although at first passage showed divergence from control, all characteristics infection recovered second sources. These included 100 % attack rates indistinguishable survival times, lesion profiles, immunohistochemical features disease-associated PrP accumulation biochemical properties. All sources, as well CH1641, transmitted to Tg338 identical clinical, pathological, features. While might potentially indicate that are strain, profound divergences evident, unable infect but lethal for mice, while reverse true combined data suggest that: (i) is stable retains its properties after sheep; (ii) it can be isolated showing a CH1641-like or more conventional phenotype; (iii) phenotype, biologically distinct experimental despite fact they behave identically single mouse line.