Autoantibodies against mouse bromelain-modified RBC are specifically inhibited by a common membrane phospholipid, phosphatidylcholine.

摘要: Sera from mice injected 4 days earlier with lipopolysaccharide lysed mouse RBC treated bromelain (brom). This lytic activity was totally inhibited by including phosphatidylcholine at final concentrations of about 2 micrograms/ml, or more, in the mixtures. In contrast, antibodies against rat not inhibited, even up to 2.5 mg/ml. Various components molecule, and other lipids closely-related molecule dipalmitoyl phosphatidyl-dimethyl-ethanolamine which is identical phosphatidylcholine, except for absence a CH2 group on polar head group, did inhibit lysis autoantibodies. Autoantibodies brom RBC, but bound to, could be eluted from, molecules attached an insoluble matrix. Liposomes prepared presence phosphatidic acid phosphatidylinositol autoantibodies same extent as liposomes only phosphatidylcholine. suggests that recognized if presented certain configuration. We suggest function these may facilitate removal membrane-damaged cells body. Such arise process ageing, because effects infectious agents such viruses.