Hemolytic anemia related to an IgM autoantibody to phosphatidylcholine that binds In vitro to stored and to bromelain-treated human erythrocytes

摘要: Both normal and autoimmune mice have IgM natural autoantibodies to bromelain-treated erythrocytes in which phosphatidylcholine (PTC) becomes exposed. At one stage this antibody may participate the genesis of hemolytic anemia NZB mouse. We recently studied a patient with who had persistently high serum titers anticardiolipin antibodies (aCL) that were also demonstrated hemolysate his obtained at time anemia. affinity-purified sought its binding human (BrE) because isotype antibody, since cardiolipin is not constituent erythrocyte wall, anionic phospholipids, aCL are known cross-react, located outer leaflet membrane. found HBrE their hemolysates by flow cytometry. cross-reacted extensively PTC, as well other or zwitterionic phospholipids. The purified lysed BrE presence complement bound vitro-aged erythrocytes. Because no evidence systemic lupus erythematosus any condition, disease represent variant described primary antiphospholipid syndrome.