[84] Pyridox(al, amine) 5′-phosphate hydrolase from rat liver

摘要: Publisher Summary This chapter describes pryridox(al, amine) 5'-phosphate hydrolase from rat liver. The tissue content of pyridoxal may be controlled by the protein binding this coenzyme and its hydrolysis a cellular phosphohydrolase. In human erythrocytes liver, phosphohydrolase activity resides in plasma membranes. methods described apply mainly to phosphate isolated membranes liver butanol solubilized enzyme same source. Pyridoxal assayed measuring either rate disappearance or release inorganic (Pi) pyridoxal. specific method employed depends upon pH, substrate concentration, other conditions experiment. Pyridoxamine is formation Pi. assays are performed under yellow light, because light-sensitive. addition these assay methods, fluorometric determination after reaction with cyanide also employed.