Microdetermination of Inorganic Phosphate, Phospholipids, and Total Phosphate in Biologic Materials
作者： E S Biologic , P P Foà , B Zak
DOI: 10.1093/CLINCHEM/13.4.326
关键词:
摘要: A rapid, reproducible, and sensitive procedure for the determination of phosphate in several biologic materials is described Organic rapidly converted to orthophosphate by digestion with a nitric acid-calcium ion mixture. The acid destroys organic material while calcium prevents loss or its conversion an unmeasurable form. Inorganic can be determined presence labile phosphates since liberated after color reaction has been initiated cannot interfere forming further color. Even if were deliberately added had started, it could not react system. method may applied present ultramicro quantities. It unnecessary extract heteropoly complex either increase sensitivity protect formed from interference hydrolyzed during process formation.
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