Validated method for quantification of gentically modified organisms in samples of maize flour
作者： Renate Kunert , Johannes S. Gach , Karola Vorauer-Uhl , Edwin Engel , Hermann Katinger
DOI: 10.1021/JF052257S
关键词: Sample preparation 、 genomic DNA 、 Genetically modified organism 、 Standard curve 、 Biology 、 Detection limit 、 Chromatography 、 DNA 、 TaqMan 、 Genetics 、 Plasmid
摘要: Sensitive and accurate testing for trace amounts of biotechnology-derived DNA from plant material is the prerequisite detection 1% or 0.5% genetically modified ingredients in food products raw materials thereof. Compared to ELISA expressed proteins, real-time PCR (RT-PCR) amplification has easier sample preparation limits are lower. Of different methods CTAB method with high flexibility starting generation sufficient relevant quality was chosen. Previous RT-PCR data generated SYBR green showed that highly sensitive matrices genomic content influencing interpretation results. Therefore, this paper describes a quantification based on TaqMan probe method, indicating accuracy sensitivity lower than 18 copies per applicable comparable purified plasmid standards as well complex samples. The results were evaluated ValiData homology variance, linearity, standard curve, deviation.
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