Transcription of simian virus 40

摘要: Asymmetric RNA was synthesized in vitro from SV40 component I DNA using Escherichia coli DNA-dependent polymerase. When denatured, unit-length, single-stranded incubated the presence of 6- to 20-fold excess asymmetric RNA, about 50% (E-DNA) formed DNA-RNA hybrids. The unhybridized (L-DNA) separated hybrids by chromatography on hydroxyapatite. E-DNA and L-DNA were shown be complementary strands DNA. After further purification shearing, hybridized extracted at different stages lytic infection transformed cells. “Early” contained sequences 30% E-strand DNA; “late” bound 30 35% Estrand 70% L-strand SV3T3 cells with 15 20%