Simian Virus 40 in Synchronized Monkey Cells: Integration of Viral DNA into the Cell Genome and Timing of Replication

摘要: Synchronized CV1, cells which were in the G1 period at time of infection, able to promote onset SV40 DNA replication during S immediately following infection. In had passed end viral did not start until next mitotic cycle. This showed that a cell controlled event, situated G1-S interval, conditioned permissiveness host genome. addition, initiation synthesis required presence least one functional gene product, as was shown through use tsA30 mutant SV40.