Induction of virus multiplication in 3T3 cells transformed by a thermosensitive mutant of polyoma virus. I. Isolation and characterization of Ts-a-3T3 cells.

摘要: Abstract Some of the biological properties 3T3 cells transformed by Ts-a , a thermosensitive mutant polyoma virus, have been studied. The maintain their phenotype if propagated at non-permissive temperature (38.5 °C); shifted to 31 °C, permissive for virus growth, variable proportion in culture produce virus. fraction productive °C increases with time as long cultures are actively growing, suggesting close association between cell growth and processes leading production. availability line which can be induced under certain conditions provides useful tool study state viral genome cells.