NUCLEOTIDE SEQUENCE OF THE MYCOBACTERIUM LEPRAE KATG REGION

摘要: Synthetic oligonucleotide primers based on the DNA sequence data of Escherichia coli, Mycobacterium tuberculosis, and intracellulare katG genes encoding heme-containing enzyme catalase-peroxidase were used to amplify analyze leprae region by PCR. A 1.6-kb fragment, which hybridized an M. tuberculosis probe, was obtained from template. Southern hybridization analysis with a probe derived PCR-amplified fragment showed that chromosome contains only one copy putative in 3.4-kb EcoRI-BamHI segment. Although nucleotide approximately 70% identical gene, no open reading frame detectable whole sequence. Moreover, two deletions 100 110 bp found region, they seemed be present all seven isolates tested. These results strongly suggest lacks functional gene activity.