Expression of Dual-Specificity Phosphatase 2 (DUSP2) in Patients with Serous Ovarian Carcinoma and in SKOV3 and OVCAR3 Cells In Vitro
作者： Wei Liu , Xiaomin Tian , Xue Ding , Leiying Zhang
DOI: 10.12659/MSM.919089
关键词: Survival analysis 、 Immunohistochemistry 、 Regulation of gene expression 、 Serous fluid 、 Cancer research 、 Malignancy 、 Ovarian carcinoma 、 Medicine 、 Dual-specificity phosphatase 、 Ovarian cancer 、 General Medicine
摘要: BACKGROUND Ovarian cancer commonly presents at a late stage and is associated with poor prognosis. The most common histological subtype serous ovarian carcinoma. Dual-specificity phosphatase 2 (DUSP2) protein substrate for mitogen-activated kinases (MAPKs) increased expression levels in malignancy. This study aimed to evaluate the of DUSP2 tumor tissues from patients carcinoma association grade, stage, patient survival investigate effects SKOV3 OVCAR3 cells vitro. MATERIAL AND METHODS Tumor tissue adjacent normal 127 histologically confirmed underwent quantitative real-time polymerase chain reaction (qRT-PCR) immunohistochemistry measure mRNA expression, respectively. clinicopathological data correlation analysis were assessed Kaplan-Meier Cox regression analysis. on proliferation migration evaluated. RESULTS Immunohistochemistry showed that was down-regulated compared tissues, significantly correlated stage. Survival an independent risk factor survival. vitro suppressed cell migration. CONCLUSIONS Down-regulation survival, its inhibited
medscimonit.com
sci-hub.st 参考文章(24)
Stephen M. Keyse, Dual-specificity MAP kinase phosphatases (MKPs) and cancer. Cancer and Metastasis Reviews. ,vol. 27, pp. 253- 261 ,(2008) , 10.1007/S10555-008-9123-1
Alexander Plotnikov, Karen Flores, Galia Maik-Rachline, Eldar Zehorai, Einat Kapri-Pardes, Denise A. Berti, Tamar Hanoch, Michal J. Besser, Rony Seger, The nuclear translocation of ERK1/2 as an anticancer target Nature Communications. ,vol. 6, pp. 6685- 6685 ,(2015) , 10.1038/NCOMMS7685
Souad Rahmouni, Fabio Cerignoli, Andres Alonso, Toshiya Tsutji, Rachel Henkens, Changjun Zhu, Christine Louis-dit-Sully, Michel Moutschen, Wei Jiang, Tomas Mustelin, Loss of the VHR dual-specific phosphatase causes cell-cycle arrest and senescence. Nature Cell Biology. ,vol. 8, pp. 524- 531 ,(2006) , 10.1038/NCB1398
Kenneth J. Livak, Thomas D. Schmittgen, ANALYSIS OF RELATIVE GENE EXPRESSION DATA USING REAL-TIME QUANTITATIVE PCR AND THE 2(-DELTA DELTA C(T)) METHOD Methods. ,vol. 25, pp. 402- 408 ,(2001) , 10.1006/METH.2001.1262
Martin Köbel, Steve E Kalloger, Niki Boyd, Steven McKinney, Erika Mehl, Chana Palmer, Samuel Leung, Nathan J Bowen, Diana N Ionescu, Ashish Rajput, Leah M Prentice, Dianne Miller, Jennifer Santos, Kenneth Swenerton, C. Blake Gilks, David Huntsman, Ovarian Carcinoma Subtypes Are Different Diseases: Implications for Biomarker Studies PLOS Medicine. ,vol. 5, ,(2008) , 10.1371/JOURNAL.PMED.0050232
Richard W Tothill, Anna V Tinker, Joshy George, Robert Brown, Stephen B Fox, Stephen Lade, Daryl S Johnson, Melanie K Trivett, Dariush Etemadmoghadam, Bianca Locandro, Nadia Traficante, Sian Fereday, Jillian A Hung, Yoke-Eng Chiew, Izhak Haviv, Australian Ovarian Cancer Study Group, Dorota Gertig, Anna DeFazio, David DL Bowtell, None, Novel Molecular Subtypes of Serous and Endometrioid Ovarian Cancer Linked to Clinical Outcome Clinical Cancer Research. ,vol. 14, pp. 5198- 5208 ,(2008) , 10.1158/1078-0432.CCR-08-0196
Xiaowen Liu, Bo Ma, Asrar B Malik, Haiyang Tang, Tao Yang, Bo Sun, Gang Wang, Richard D Minshall, Yan Li, Yong Zhao, Richard D Ye, Jingsong Xu, Bidirectional regulation of neutrophil migration by mitogen-activated protein kinases. Nature Immunology. ,vol. 13, pp. 457- 464 ,(2012) , 10.1038/NI.2258
Yke Jildouw Arnoldussen, Petra I. Lorenzo, Maria E. Pretorius, Håkon Wæhre, Bjørn Risberg, Gunhild M. Mælandsmo, Håvard E. Danielsen, Fahri Saatcioglu, The Mitogen-Activated Protein Kinase Phosphatase Vaccinia H1-Related Protein Inhibits Apoptosis in Prostate Cancer Cells and Is Overexpressed in Prostate Cancer Cancer Research. ,vol. 68, pp. 9255- 9264 ,(2008) , 10.1158/0008-5472.CAN-08-1224
Tanja Haag, Antje M. Richter, Martin B. Schneider, Adriana P. Jiménez, Reinhard H. Dammann, The dual specificity phosphatase 2 gene is hypermethylated in human cancer and regulated by epigenetic mechanisms. BMC Cancer. ,vol. 16, pp. 49- 49 ,(2016) , 10.1186/S12885-016-2087-6
Hongda Liu, Qun Zhang, Kangshuai Li, Zheng Gong, Zhaochen Liu, Yunfei Xu, Mary Hannah Swaney, Kunhong Xiao, Yuxin Chen, Prognostic significance of USP33 in advanced colorectal cancer patients: new insights into β-arrestin-dependent ERK signaling. Oncotarget. ,vol. 7, pp. 81223- 81240 ,(2016) , 10.18632/ONCOTARGET.13219