Morphological Characterization of Kupffer and Endothelial Cells of Rat Liver Isolated By Counterflow Elutriation
作者： Rainer N. Zahlten , Herbert K. Hagler , Michael E. Nejtek , C. Jeffrey Day
DOI: 10.1016/0016-5085(78)93769-1
关键词: Cell biology 、 Biology 、 Elutriation 、 Collagenase 、 Liver cytology 、 Endothelium 、 Metrizamide 、 Microscopy 、 Immunohistochemistry 、 Pathology 、 Rat liver
摘要: Kupffer and endothelial cells of rat liver were purified by recirculating collagenase perfusion, metrizamide gradient, counterflow elutriation. Every step the isolation procedure was followed light microscopy histochemistry. The final fractions sinusoidal examined in their isolated state transmission scanning electron microscopy. scope this manuscript is detailed documentation discussion all technical aspects problems a rather new technique, as well description Broader acceptance should lead to specific characterization biochemical immunological functions these elucidate pathophysiological significance.
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sci-hub.st 参考文章(22)
Michielsen Ce, Jones Al, Schmucker Dl, An improved system for hemoglobin-free perfusion of isolated rat livers. Laboratory Investigation. ,vol. 33, pp. 168- ,(1975)
H. D. Fahimi, J. J. Widmann, Proliferation of mononuclear phagocytes (Kupffer cells) and endothelial cells in regenerating rat liver. A light and electron microscopic cytochemical study. American Journal of Pathology. ,vol. 80, pp. 349- 366 ,(1975)
R.J. Sanderson, Karyn E. Bird, Chapter 1 Cell Separations by Counterflow Centrifugation. Methods in Cell Biology. ,vol. 15, pp. 1- 14 ,(1977) , 10.1016/S0091-679X(08)60206-X
P.E. Lentz, N.R. Di Luzio, [64] Isolation of adult rat liver macrophages (Kupffer cells) Biomembranes Part B. ,vol. 32, pp. 647- 653 ,(1974) , 10.1016/0076-6879(74)32067-8
Hans Ulrich Bergmeyer, Methods of Enzymatic Analysis ,(1963)
Toshio Ito, Manji Nemoto, ^|^Uuml;ber die Kupfferschen Sternzellen und die ^|^ldquo;Fettspeicherungszellen^|^rdquo; (^|^ldquo;fat storing cells^|^rdquo;) in der Blutkapillarenwand der menschlichen Leber. Okajimas Folia Anatomica Japonica. ,vol. 24, pp. 243- 258 ,(1952) , 10.2535/OFAJ1936.24.4_243
D. M. Mills, D. Zucker-Franklin, Electron microscopic study of isolated Kupffer cells. American Journal of Pathology. ,vol. 54, pp. 147- 166 ,(1969)
E. Wisse, Observations on the fine structure and peroxidase cytochemistry of normal rat liver Kupffer cells Journal of Ultrastructure Research. ,vol. 46, pp. 393- 426 ,(1974) , 10.1016/S0022-5320(74)90064-1
S.R. Wagle, Friedrich Hofmann, Karl Decker, Studies on the stimulation of gluconeogenesis and lipolysis by glucagon and epinephrine in isolated rat Kupffer cells Biochemical and Biophysical Research Communications. ,vol. 71, pp. 857- 863 ,(1976) , 10.1016/0006-291X(76)90910-4
E. Wisse, J. M. van't Noordende, J. van der Meulen, W. Th. Daems, The pit cell: Description of a new type of cell occurring in rat liver sinusoids and peripheral blood Cell and Tissue Research. ,vol. 173, pp. 423- 435 ,(1976) , 10.1007/BF00224305