An improved system for hemoglobin-free perfusion of isolated rat livers.

摘要: Fine structure and several parameters of hepatic function were evaluated in isolated rat livers perfused with a hemoglobin-free medium newly designed organ perfusion system. The preservation fine the maintenance certain functional criteria, including oxygen consumption, bile secretion rate, retention intracellular potassium ions, demonstrated that these had been successfully for up to 5 hours. contained neither red blood cells nor other carriers, such as fluorocarbon emulsions. use hyperbaric oxygenation eliminates requirement hemoglobin medium. In addition, extensive endothelial cell injury associated media is avoided since this system permits at near physiologic portal vein flow rates. quality without albumin suggests an oncotic agent not required liver perfusion.