THE DEPENDENCE OF HEPATIC FUNCTION UPON SUFFICIENT OXYGEN SUPPLY DURING PROLONGED ISOLATED RAT LIVER PERFUSION

摘要: It is unclear how the omission of a perfusate oxygen carrier may influence data derived from prolonged in vitro liver perfusions. The effects reduced content upon function tests conducted livers male Sprague-Dawley rats perfused for 6 h, were studied using diluted rat blood (PCV = 10%) (group 1) and compared to lines with Krebs' buffer 2). Perfusate bile samples withdrawn at start hourly intervals following commencement perfusion biochemical analysis aspartate-serine transaminase, alkaline phosphatase, bilirubin, urea potassium concentrations, also gas measurements calculations hepatic uptake. There no significant differences between initial pressures 11.4+/-1.6 15.7+/-2.4 mm Hg 1 versus group 2, respectively). Maintenance comparable perfusion, however, resulted difference fixed flow rate two groups 1.9+/-0.1 2.6+/-0.2* ml/min/g significantly higher 2 than after h (18.2+/-2.0 8.7+/-1.7 mmHg, Bile volume production was greater 784+/-84 vs 458+/-75 microl/h** respectively. Hepatic uptake (HOU) maximal 0.93+/-0.13 0.32+/-0.08** micromol/min/g, concentrations bilirubin 23.0+/-0.6 4.9+/-0.4 micromol/l*** 4.7+/-0.5 1.1+/-0.2 mmol/l**, urea, glucose 5.7+/-0.5 1.4+/-0.2 micromol/l**; 2.4+/-0.5 0.7+/-0.1 micromol/l** 22.1+/-1.4 17.8+/-0.9 micromol/l***. concluded that Krebs'-Henseleit (KHB) via portal vein alone be insufficient maintain optimum as assessed by used. Inclusion optimal if are physiological pressures. *p < 0.05; **p 0.01; ***p 0.001; Student's unpaired t test.