Age-dependent changes of biochemical parameters during the perfusion of the isolated rat liver

作者: T. Vömel

DOI: 10.1016/0047-6374(85)90094-6

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摘要: The livers were isolated from Sprague-Dawley rats in 5 groups aged 4 to 5, 10 12, 24 26, 30 32 and 35 37 months perfused for h study the activities of GOT GPT, K+ level form quotient lactate/pyruvate beta-hydroxybutyrate/acetoacetate perfusion medium. showed no age related changes, which eliminates, by use method described this paper, as sensitive parameter cell membrane function. Both redox quotients indicate a metabolic breakdown after 240 min oldest group, points reduced adaptability senescent animals changed metabolical conditions during livers. transaminases GPT proved be most parameters function, allowing finest discriminations between various groups. Here also group first signs damages.

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