Surface Immobilization of Synthetic Proteins Via Plasma Polymer Interlayers

摘要: Coatings of biologically active molecules on synthetic ”bulk“materials are much interest for biomedical applications since they can in principle elicit specific, predictable. controlled responses the host environment to an implanted device. However, issues such as shelf life. storage conditions, biological safety, and enzymatic attack must be considered; proteins may offer advantages. In this study we investigated covalent immobilization onto polymeric materials which possess some properties that mimic those natural protein collagen, particularly ability form triple helical structures, thus provide similar bio-responses while avoiding degradation. order perform these collagen-like (CLMs) under mild reaction bulk first equipped with suitable surface groups using rf plasma methods. Plasma polymer interlayers advantages versatile reactive platforms other molecules. Application a thin coating from aldehyde monomer is it enables direct CLMs by their terminal amine groups, reductive amination chemistry. An alternative route via layers contain carboxylic acid carbodiimnide A third makes use alkylamme interlayers, less process sensitive than coatings. layer poly-carboxylic compounds terminated PAMAM-starburst dendrimers or carboxymethylated dextran then attached carbodiimide chemistry layer. Amine-terminated immobilized Surface analytical methods have been used characterize steps assess coverage. Initial cell attachment growth assays indicate performance depends amino sequence.