Sensitive SERS glucose sensing in biological media using alkyne functionalized boronic acid on planar substrates

摘要: In this work, we propose a novel glucose binding mechanism on highly sensitive SERS substrate, in order to overcome challenges specific detection bio-fluids. We make use of phenylboronic acid as receptor for saccharide capture onto the substrate and ability captured molecule undergo secondary with an alkyne-functionalized boronic form glucose-alkyne-boronic complex. The formation complex shows high selectivity glucose, over other saccharides. addition, alkyne group exhibits distinct Raman peak at 1996 cm(-1) biological silent region (1800-2800 cm(-1)) where most endogenous molecules, including show no scattering, thus offering sensitivity sensing. offers long-term stability, well enhancement glucose-alkyne substrate. reversibility signals various incubation stages also reusability capabilities, whereas positive results clinical urine samples demonstrate feasibility. All these strongly suggest that newly developed SERS-based assay great potential