Augmentation of type I IL-1 receptor expression and IL-1 signaling by IL-6 and glucocorticoid in murine hepatocytes.
作者： Takemasa Takii , Atsushi Ito , Kikuo Onozaki , Takayuki Matsumura
DOI:
关键词: Molecular biology 、 Endocrinology 、 Amyloid 、 Receptor expression 、 Dexamethasone 、 Cell 、 Glucocorticoid 、 Internal medicine 、 Interleukin 6 、 In vivo 、 Biology 、 Kinase
摘要: IL-1 signal is transduced through type I receptor (IL-1RI). We have recently reported that LPS augments IL-1RI mRNA expression in the hepatocytes of mice vivo, and augmentation mediated by interaction IL-1, IL-6, glucocorticoid (GC). In this study, we examined whether level reflects those cell surface molecule signaling. When primary cultured murine were treated with dexamethasone (Dex) or these two reagents synergistically up-regulated cells. 125I-labeled binding experiment showed was also treatment Dex IL-6. Scatchard analysis revealed number IL-1R increased. The increased completely inhibited an Ab against IL-1RI, indicating IL-6 augmented molecule. pretreated activation IL-1R-associated kinase response to signaling augmented. addition, following administration combination into markedly serum amyloid A. These results indicate GC augment hepatocytes, as well activation, up-regulation level, which represents a novel regulatory network between GC,
jimmunol.org 参考文章(62)
L K Birinyi, P Libby, K R Auger, J M Ordovas, A H Robbins, C A Dinarello, Endotoxin and tumor necrosis factor induce interleukin-1 gene expression in adult human vascular endothelial cells. American Journal of Pathology. ,vol. 124, pp. 179- 185 ,(1986)
CM Dubois, FW Ruscetti, SE Jacobsen, JJ Oppenheim, JR Keller, Hematopoietic growth factors upregulate the p65 type ii interleukin 1 receptor on bone marrow progenitor cells in vitro Blood. ,vol. 80, pp. 600- 608 ,(1992) , 10.1182/BLOOD.V80.3.600.600
CA Dinarello, Biologic basis for interleukin-1 in disease Blood. ,vol. 87, pp. 2095- 2147 ,(1996) , 10.1182/BLOOD.V87.6.2095.BLOODJOURNAL8762095
L Rawlinson, M.R. Edbrooke, P Woo, J Saklatvala, E Stylianou, L.A. O'Neill, Interleukin 1 induces NF-kappa B through its type I but not its type II receptor in lymphocytes. Journal of Biological Chemistry. ,vol. 267, pp. 15836- 15841 ,(1992) , 10.1016/S0021-9258(19)49611-7
A S Whitehead, R B Sim, J Herbert, S Grehan, C M Uhlar, Expression of a biologically active recombinant mouse IL-1 receptor antagonist and its use in vivo to modulate aspects of the acute phase response. Journal of Immunology. ,vol. 159, pp. 369- 378 ,(1997)
N. Auphan, J. A. DiDonato, C. Rosette, A. Helmberg, M. Karin, Immunosuppression by Glucocorticoids: Inhibition of NF-κB Activity Through Induction of IκB Synthesis Science. ,vol. 270, pp. 286- 290 ,(1995) , 10.1126/SCIENCE.270.5234.286
W J Chiou, P D Bonin, P K W Harris, D B Carter, J P Singh, Platelet-derived growth factor induces interleukin-1 receptor gene expression in Balb/c 3T3 fibroblasts. Journal of Biological Chemistry. ,vol. 264, pp. 21442- 21445 ,(1989) , 10.1016/S0021-9258(20)88199-X
P D Bonin, J P Singh, Modulation of interleukin-1 receptor expression and interleukin-1 response in fibroblasts by platelet-derived growth factor. Journal of Biological Chemistry. ,vol. 263, pp. 11052- 11055 ,(1988) , 10.1016/S0021-9258(18)37917-1
F. Colotta, F. Re, M. Muzio, N. Polentarutti, A. Minty, D. Caput, P. Ferrara, A. Mantovani, Interleukin-13 induces expression and release of interleukin-1 decoy receptor in human polymorphonuclear cells. Journal of Biological Chemistry. ,vol. 269, pp. 12403- 12406 ,(1994) , 10.1016/S0021-9258(18)99886-8
D Pennica, D V Goeddel, P W Gray, D Glaister, E Chen, Two interleukin 1 genes in the mouse: cloning and expression of the cDNA for murine interleukin 1 beta. Journal of Immunology. ,vol. 137, pp. 3644- 3648 ,(1986)