Ripening of Ossau-Iraty cheese: determination of free amino acids by RP-HPLC and of total free amino acids by the TNBS method.

作者: J.M Izco , P Torre , Y Barcina

DOI: 10.1016/S0956-7135(99)00031-6

关键词: RipeningValineChemistryAmino acidPhenylalanineCheese ripeningLeucineAsparagineGlutamineChromatography

摘要: Abstract The free amino acids and sulphosalicylic acid-soluble N fraction in the French ewes’-milk cheese, Ossau-Iraty, were determined to evaluate degree of proteolysis during ripening cheese. RP-HPLC was used assess acids, trinitrobenzenesulphonic acid method fraction. total content as by ranged from 200 mg/100 g dry matter on first day 2200 after 120 d ripening. major glutamic acid, asparagine, glutamine, valine, leucine, phenylalanine lysine, which accounted for between 50% (on 1) 70% 120) acids. Correlations all main time good. Regression yielded a correlation coefficient value 0.985.

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