Accelerated ripening of Manchego type cheese by added commercial enzyme preparation from Aspergillus oryzae

作者: Estrella Fernandez-Garcia , Agustin Olano , Dolores Cabezudo , Pedro J. Martin-Alvarez , Mercedes Ramos

DOI: 10.1016/0141-0229(93)90086-H

关键词:

摘要: Abstract The effect of the addition an enzyme preparation from Aspergillus oryzae (Flavor Age) on proteolysis and sensory characteristics Manchego type cheese made a mixture ewes' cows' milk was studied. Proteolysis, as measured by noncasein nitrogen, trichloroacetic acid-soluble sulfosalicylic free amino acids, polyacrylamide gel electrophoresis (PAGE) casein fraction, increased adding enzyme. Neither bitter taste nor off-flavors were detected in concentration range used (25–50 mg kg−1 milk). Sensory analysis showed significant differences between estimated age real ripening time cheeses with 50 Flavor Age, significantly higher-quality scores for added

参考文章(19)
E. Fernandez-Garcia, R. Lopez-Fandino, M. Ramos, A. Olano, Comparative study of the proteolytic activity of a Bacillus subtilis neutral protease preparation during early stages of ripening of cheeses made from cow and ewe milk. Milchwissenschaft-milk Science International. ,vol. 45, pp. 428- 431 ,(1990)
S.C. Silver, J.T. Zelko, P.R. Freund, M.V. Arbige, Novel lipase for cheddar cheese flavor development Food Technology. ,vol. 40, pp. 91- 98 ,(1986)
Robert W. Blakesley, John A. Boezi, A new staining technique for proteins in polyacrylamide gels using coomassie brilliant blue G250. Analytical Biochemistry. ,vol. 82, pp. 580- 582 ,(1977) , 10.1016/0003-2697(77)90197-X
Robyn M. Hillier, The quantitative measurement of whey proteins using polyacrylamide-gel electrophoresis Journal of Dairy Research. ,vol. 43, pp. 259- 265 ,(1976) , 10.1017/S0022029900015818
V.K. Sood, F.V. Kosikowski, Accelerated Cheddar Cheese Ripening by Added Microbial Enzymes Journal of Dairy Science. ,vol. 62, pp. 1865- 1872 ,(1979) , 10.3168/JDS.S0022-0302(79)83516-X
M. Ramos, I. Martinez-Castro, M. Juarez, Detection of Cow's Milk in Manchego Cheese Journal of Dairy Science. ,vol. 60, pp. 870- 877 ,(1977) , 10.3168/JDS.S0022-0302(77)83956-8
S. M. Farahat, A. M. Rabie, A. A. Abdel Baky, A. A. El-Neshawy, S. Mobasher, β-Galactosidase in the acceleration of Ras cheese ripening Nahrung-food. ,vol. 29, pp. 247- 254 ,(1985) , 10.1002/FOOD.19850290307
J.C.C. LIN, I.J. JEON, H.A. ROBERTS, G.A. MILLIKEN, Effects of Commercial Food Grade Enzymes on Proteolysis and Textural Changes in Granular Cheddar Cheese Journal of Food Science. ,vol. 52, pp. 620- 625 ,(1987) , 10.1111/J.1365-2621.1987.TB06688.X
Rosina López-Fandiño, Mercedes Ramos, Estrella Fernández-García, Agustin Olano, Proteolytic activity of two commercial proteinases from Aspergillus oryzae and Bacillus subtilis on ovine and bovine caseins. Journal of Dairy Research. ,vol. 58, pp. 461- 467 ,(1991) , 10.1017/S0022029900030065
J. P. PÉLISSIER, P. MANCHON, COMPARATIVE STUDY OF THE BITTER TASTE OF ENZYMIC HYDROLYSATES FROM COW, EWE AND GOAT CASEINS Journal of Food Science. ,vol. 41, pp. 231- 233 ,(1976) , 10.1111/J.1365-2621.1976.TB00590.X