Glycoprotein K of herpes simplex virus: a transmembrane protein encoded by the UL53 gene which regulates membrane fusion.

摘要: Glycoprotein K (gK) encoded by the UL53 gene is ninth out of eleven HSV glycoproteins (gps). The precursor gK (pgK) a transmembrane protein with four hydrophobic domains, which consists 338 amino acids. has two initiation codons: upper overlaps UL52 ORF, while lower located 55 codons downstream and specifies truncated polypeptide. upstream have common polyadenylation signal from stop codon so that mRNA completely nested within transcript. syn1 mutations in several KOSsyn mutants MPsyn virus, had been fine mapped to DNA coordinates 0.735–0.740, were later on gene, especially its portion first 120 acids (aa) N-terminus (most frequently residue 40) less precisely defined locus between aa 301–310 (close C-terminus). Point N-terminal ectodomain gK, are related syn formation, impair putative ability this region down-regulate membrane fusion. N-glycosylated mannose core oligosaccharides attached Asn residues polypeptide at positions 48 58, respectively. In infected cells, localized mainly nuclear endoplasmic reticulum (ER) membranes. It not clear, whether becomes incorporated into envelope mature particles. Studies insertion/deletion showed importance for capsid envelopment, transportation egress or virions cells. seems an essential role virion egress, even though glycoprotein acts accord gH another UL20 gene.