Sizing Channels with Neutral Polymers

作者: O. V. Krasilnikov

DOI: 10.1007/978-94-010-0401-5_6

关键词: Ethylene glycolNanometreChemical engineeringConductivityMaterials scienceIonic bondingPolymerIon channelPEG ratioConductance

摘要: We have developed a method for determining the physical dimensions of nanometer scale pores formed by protein ion channels. This was possible because availability wide range size-selected nonelectrolyte polymers poly(ethylene glycol), PEG, and PEG decreases bulk conductivity ionic solutions. The is simple. PEGs that are sufficiently small enter channel’s pore decrease conductance. larger than pore’s diameter rarely partition into therefore do not channel Thus, dependence conductance on molecular weight determines cut-off, inference, radius. recently extended technique to determine shape lumen including sizes both openings size location constrictions inside pore. discuss here details method, properties some limitations using size.

参考文章(62)
Kris P. Antonsen, Allan S. Hoffman, Water Structure of PEG Solutions by Differential Scanning Calorimetry Measurements Poly(Ethylene Glycol) Chemistry. pp. 15- 28 ,(1992) , 10.1007/978-1-4899-0703-5_2
T Tsukihara, Membrane protein assemblies — towards atomic resolution analysis Commentary Current Opinion in Structural Biology. ,vol. 10, pp. 208- 212 ,(2000) , 10.1016/S0959-440X(00)00069-5
A. Valeva, A. Weisser, B. Walker, M. Kehoe, H. Bayley, S. Bhakdi, M. Palmer, MOLECULAR ARCHITECTURE OF A TOXIN PORE : A 15-RESIDUE SEQUENCE LINES THE TRANSMEMBRANE CHANNEL OF STAPHYLOCOCCAL ALPHA -TOXIN The EMBO Journal. ,vol. 15, pp. 1857- 1864 ,(1996) , 10.1002/J.1460-2075.1996.TB00536.X
J. V. Koleske, Frederick E. Bailey, Poly(ethylene oxide) Academic Press. ,(1976)
J. V. Koleske, Frederick E. Bailey, Alkylene Oxides and Their Polymers ,(1990)
J.C. Lee, L.L. Lee, Preferential solvent interactions between proteins and polyethylene glycols. Journal of Biological Chemistry. ,vol. 256, pp. 625- 631 ,(1981) , 10.1016/S0021-9258(19)70019-2
Rene Scherrer, Philipp Gerhardt, Molecular Sieving by the Bacillus megaterium Cell Wall and Protoplast Journal of Bacteriology. ,vol. 107, pp. 718- 735 ,(1971) , 10.1128/JB.107.3.718-735.1971