Label-Free, Multiplex Glycan Microarray Biosensor for Influenza Virus Detection.

摘要: Newly emerging influenza viruses adapted from animal species pose significant pandemic threats to public health. An understanding of hemagglutinin (HA) receptor-binding specificity host receptors is key studying the adaptation in humans. This information may be particularly useful for predicting emergence a outbreak. Therefore, high-throughput sensing technologies able profile HA receptor binding can facilitate studies virus evolution and As step toward this goal, we have prepared glycan-based analogue microarrays on Arrayed Imaging Reflectometry (AIR) platform. These arrays demonstrate label-free, multiplex detection discrimination between human avian viruses. Microarrays consisting glycan probes with 2,6 2,3 linkages were prepared. After first confirming their ability capture lectins (carbohydrate-binding proteins) known specificities, observed that discriminate quantify A/California/07/2009 (H1N1pdm) A/Netherlands/1/2000 (H13N8) viruses, respectively. method expanded large numbers glycans (>100) subtypes (H1-H18), anticipate it applied systematically evaluate In turn, will global surveillance serve as new tool enabling health organizations, governments, research institutes, laboratories react quickly face