Protein analysis of defective interfering lymphocytic choriomeningitis virus and persistently infected cells.

摘要: Abstract Defective interfering (DI) lymphocytic choriomeningitis virus (LCMV) was purified from the culture fluids of BHK 21/13S and L-929 cells persistently infected with LCMV. DI LCMV sedimented in renografin-76 gradients to a density slightly less than standard (S) (1.13 vs 1.14 g/cm 3 ). Polyacrylamide gel electrophoretic analysis [ 35 S]methionine-labeled revealed major 63,000-dalton polypeptide corresponding S virion nucleoprotein (NP), two minor polypeptides 54,000− 35,000-dalton glycopeptides. No differences composition were detected between virions. Exposure before challenge inhibited intracellular synthesis NP. Cells released no detectable or temperature-sensitive mutants but did release The production by these cultures 10− 100-fold lower during acute infections. These contained intracytoplasmic NP, immunofluorescence, its rate too low be radiolabeling methods used. Although present cytoplasms, viral antigens absent cell membranes many cells. Thus, produce relatively levels which can inhibit protein synthesis. factors may act render resistant immunosurveillance mechanisms persistent infections vivo .