Cotransplantation of Mesenchymal Stem Cells With Neonatal Porcine Islets Improve Graft Function in Diabetic Mice

摘要: Mesenchymal stem cells (MSCs) possess immunoregulatory, anti-inflammatory, and proangiogenic properties and, therefore, have the potential to improve islet engraftment survival. We assessed effect human bone marrow–derived MSCs on neonatal porcine islets (NPIs) in vitro determined metabolic outcomes when cotransplanted a mouse model. NPIs cocultured with had greater cellular insulin content increased glucose-stimulated secretion. were or without diabetic B6.129S7-Rag1 tm1Mom /J mice. Blood glucose weight monitored until reversal of diabetes; mice then given an oral tolerance test. Islet grafts for degree vascularization total content. Cotransplantation resulted significantly earlier normoglycemia vascularization, improved tolerance, One experiment conducted from donor autoimmune disorder no positive effects transplant outcomes. demonstrated beneficial likely as result engraftment. In addition, pathology can influence functional capacity MSCs.