Protective Effect of Chokeberry Anthocyanin-rich Fraction at Nanomolar Concentrations against Oxidative Stress Induced by High Doses of Glucose in Pancreatic β-cells

摘要: The purposes of this study was to evaluate the protective action nanomolar concentrations an anthocyanin rich fraction separated from chokeberry, against oxidative stress induced by high doses glucose (50 and 100 mM) in pancreatic β-cells. Using different (from 0.2 3 nM), we evaluated its role mouse β-cells (TC-3), measuring viability, intracellular reactive oxygen species (ROS) levels glutathione. Our results indicate scavenging effect chokeberry anthocyanins 1.5 when on ROS species, which were quenched, according decrease DCF fluorescence. Also, 1.0 nM, presence mM proved restore, proportionally dose, strong glutathione (induced high-glucose), as compared untreated cells.