Effects of co-supplementation of vitamins E and C on gentamicin-induced nephrotoxicity in rat.

摘要: Gentamicin (GM) is an effective antibiotic against severe gram-negative infections. However it can produce nephrotoxicity in human. Reactive oxygen species (ROS) have been proposed as the causative factors of renal side effects drug. This study was performed to investigate protective role antioxidant vitamins GM-mediated nephropathy situ model isolated rat kidney. Male Sprague-Dawley rats were randomly assigned one following groups seven rats: group 1 (Control) perfused with Tyrode solution; 2 (GM), 200 μg ml−1 GM added perfusate; 3 (GM + Vit C), vitamin C drinking water for days (200 mg l−1) and perfusate (100 l−1); 4 E), E g body weight)−1, i.m.) injected 12 h before start experiment; 5 E) co-administered (concentrations conditions 4). To compare groups, urinary lactate dehydrogenase (LDH), N-acetyle-β-d-glucosaminidase (NAG) alkaline phosphatase (ALP) activities, inulin clearance (glomerular filtration rate, GFR) tissue glutathione (GSH) content measured. caused a significant demonstrated by increase LDH, NAG ALP activities. Reduction GSH marked decrease GFR observed compared controls. Vitamin inhibited GM-induced enzyme activities but did not show effect on or GFR. prevented reduction lelvel without improvement Co-administration significantly demonstrating preservation levels prevention We conclude that co-administration moderate doses has beneficial nephrotoxicity.