Adaptation of the bivalve embryotoxicity assay for the high throughput screening of emerging contaminants in Mytilus galloprovincialis

摘要: Abstract Emerging contaminants (such as Endocrine disrupting chemicals-EDCs, brominated and perfluorinated compounds-BFRs PFCs, pharmaceuticals) are chemicals currently not included in regulatory monitoring programs, whose fate biological impacts poorly understood. Assessment of ecosystem health with respect to these is particular concern also the marine environment: this respect, data on effects early life stages important establish sensitivity species. In work, acute (48 h) bivalve embryo toxicity test was applied for screening developmental different emerging Mediterranean mussel Mytilus galloprovincialis . The assay adapted 96-microwell plates, standardized order obtain normal D-shaped larvae acceptability results based negative control positive (copper) comparable those reported literature spp. model compounds representative EDCs (Nonylphenol-NP Bisphenol A-BPA), BFRs (Tetrabromobisphenol A-TBBPA), PFCs (perfluorooctanoid acid-PFOA perfluorooctane sulphonate-PFOAS) pharmaceuticals (Ibuprofen-IBU, Diclofenac-DCF, Bezafibrate-BEZA) a wide concentration range (0.01–0.1–1–10–100–1000 μg/L) were evaluated. proved sensitive tool high throughput species, leading production significant amounts that may be useful purposes.