Structural investigation of carboxymethyl cellulose biodeterioration by Bacillus subtilis subsp. subtilis NCIB 3610
作者： Janez Orehek , Iztok Dogsa , Matija Tomšič , Andrej Jamnik , Drago Kočar
DOI: 10.1016/J.IBIOD.2012.10.007
关键词: Carboxymethyl cellulose 、 Bacillus subtilis 、 Small-angle X-ray scattering 、 Viscosity 、 Size-exclusion chromatography 、 Hydrolysis 、 Chemical engineering 、 Chemistry 、 Radius of gyration 、 Cellulase 、 Chromatography
摘要: Abstract Structural changes of carboxymethyl cellulose (CMC) during biodeterioration with Bacillus subtilis subsp . NCIB 3610 were studied small-angle X-ray scattering (SAXS), size exclusion chromatography, and viscosity measurements. Cleavage CMC polymers changed flow characteristics from those non-Newtonian pseudoplastic fluid to Newtonian fluid. The decreased 10 1.4 mPas the concentration reducing sugar increased by 18-fold. Upon average radius gyration 147 123 A; cleaved polymer chains remained in close contact unstirred medium as reflected a relatively large apparent gyration. However, such degraded aggregates easily dispersed shear stress. results suggest that mainly affects structural features are larger than 5 nm. Furthermore, also metabolism may be decoupled; B. produces cellulases effectively cleave but cannot efficiently utilize hydrolyzed products. There no or rheological observed absence growth.
sci-hub.se 参考文章(48)
Elwyn T. Reese, Ralph G. H. Siu, Hillel S. Levinson, The biological degradation of soluble cellulose derivatives and its relationship to the mechanism of cellulose hydrolysis. Journal of Bacteriology. ,vol. 59, pp. 485- 497 ,(1950) , 10.1128/JB.59.4.485-497.1950
Roger Gabriel, Glossary of technical terms Springer, Dordrecht. pp. 13- 26 ,(1980) , 10.1007/978-94-015-7148-7_2
L. D. Bushnell, H. F. Haas, The Utilization of Certain Hydrocarbons by Microorganisms 1 Journal of Bacteriology. ,vol. 41, pp. 653- 673 ,(1941) , 10.1128/JB.41.5.653-673.1941
Henry Black, Determination of Sodium Carboxymethylcellulose in Detergent Mixtures by Anthrone Method Analytical Chemistry. ,vol. 23, pp. 1792- 1795 ,(1951) , 10.1021/AC60060A021
Michel. DuBois, K. A. Gilles, J. K. Hamilton, P. A. Rebers, Fred. Smith, Colorimetric Method for Determination of Sugars and Related Substances Analytical Chemistry. ,vol. 28, pp. 350- 356 ,(1956) , 10.1021/AC60111A017
Ramesh Chand Kasana, Richa Salwan, Hena Dhar, Som Dutt, Arvind Gulati, A rapid and easy method for the detection of microbial cellulases on agar plates using gram's iodine Current Microbiology. ,vol. 57, pp. 503- 507 ,(2008) , 10.1007/S00284-008-9276-8
U. Kasulke, K.-J. Linow, B. Philipp, H. Dautzenberg, Untersuchungen zum hydrolytischen abbau wasserlöslicher cellulosederivate unter homogenen reaktionsbedingungen. 4. Mitteilung: Mechanismus der enzymatischen hydrolyse Acta Polymerica. ,vol. 39, pp. 127- 130 ,(1988) , 10.1002/ACTP.1988.010390308
A. Bär, B. Van Ommen, M. Timonen, Metabolic disposition in rats of regular and enzymatically depolymerized sodium carboxymethylcellulose Food and Chemical Toxicology. ,vol. 33, pp. 901- 907 ,(1995) , 10.1016/0278-6915(95)00068-D
N. Diftis, V. Kiosseoglou, Improvement of emulsifying properties of soybean protein isolate by conjugation with carboxymethyl cellulose Food Chemistry. ,vol. 81, pp. 1- 6 ,(2003) , 10.1016/S0308-8146(02)00236-4
Huarong Nie, Mingzhu Liu, Falu Zhan, Mingyu Guo, Factors on the preparation of carboxymethylcellulose hydrogel and its degradation behavior in soil Carbohydrate Polymers. ,vol. 58, pp. 185- 189 ,(2004) , 10.1016/J.CARBPOL.2004.06.035