The effect of chemical modification of 3-(3-amino-3-carboxypropyl)uridine on tRNA function.
作者： S Friedman
DOI: 10.1016/S0021-9258(18)50291-X
关键词: Protein tertiary structure 、 Transfer RNA 、 Phenylalanine 、 Chemical modification 、 Uridine 、 Stereochemistry 、 Chemistry 、 Acylation 、 Ribosome 、 Escherichia coli
摘要: Abstract The minor base 3-(3-amino-3-carboxypropyl)uridine (acp3U) in Escherichia coli tRNAPhe was acylated with the N-hydroxysuccinimide esters of acetic, phenoxy-acetic, and naphthoxyacetic acid, as well ester 5-dimethylaminonaphthalene-1-sulfonyl (dansyl)-glycine. derivatives formed were all capable accepting phenylalanine. There only effects on kinetic parameters these for E. phenylalanyl-tRNA synthetase. no effect ability to participate poly(U)- or poly(ACU)-directed polypeptide synthesis poly(U)-stimulated binding ribosomes. rate photodynamic cross-linking 4-Srd 8 Cyd 13 decreased tRNAs containing acetyl dansyl-glycyl acp3U, indicating that acylation this may perturb tertiary structure tRNA. This does not appear play any role known biological functions tRNAPhe.
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