Sensitivity of digoxigenin and biotin labelled probes for detection of human papillomavirus by in situ hybridisation.
作者： R G Morris , M J Arends , P E Bishop , K Sizer , E Duvall
关键词: Primer (molecular biology) 、 Nick translation 、 Hybridization probe 、 Digoxigenin 、 Streptavidin 、 Biology 、 Biotinylation 、 Molecular biology 、 Biotin 、 Nucleic acid thermodynamics
摘要: … Abstract The sensitivity of digoxigenin and biotin labelled … with anti-digoxigenin antibody, or labelled with biotin by nick … Digoxigenin labelled probes proved two to 10-fold more sen…
core.ac.uk
ed.ac.uk
sci-hub.se 参考文章(22)
M Thomas-Cavallin, O Aït-Ahmed, The random primer labeling technique applied to in situ hybridization on tissue sections. Journal of Histochemistry and Cytochemistry. ,vol. 36, pp. 1335- 1340 ,(1988) , 10.1177/36.10.3138309
F A Lewis, S Griffiths, R Dunnicliff, M Wells, N Dudding, C C Bird, Sensitive in situ hybridisation technique using biotin-streptavidin-polyalkaline phosphatase complex. Journal of Clinical Pathology. ,vol. 40, pp. 163- 166 ,(1987) , 10.1136/JCP.40.2.163
G S Wood, R Warnke, Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems. Journal of Histochemistry and Cytochemistry. ,vol. 29, pp. 1196- 1204 ,(1981) , 10.1177/29.10.7028859
C. P. Crum, Nobutaka Nagai, S. Silverstein, R. U. Levine, In situ hybridization analysis of HPV 16 DNA sequences in early cervical neoplasia. American Journal of Pathology. ,vol. 123, pp. 174- 182 ,(1986)
E. Genersch, H. J. Eggers, R. Neumann, C. Kessler, H. B. J. Heiles, In situ hybridization with digoxigenin-labeled DNA of human papillomaviruses (HPV 16/18) in HeLa and SiHa cells. BioTechniques. ,vol. 6, pp. 978- 981 ,(1988)
Alex Ferenczy, Lundy Braun, Keerti V. Shah, Human papillomavirus (HPV) in condylomatous lesions of cervix. A comparative ultrastructural and immunohistochemical study The American Journal of Surgical Pathology. ,vol. 5, pp. 661- 670 ,(1981) , 10.1097/00000478-198110000-00008
Herbert Pfister, Biology and biochemistry of papillomaviruses. Reviews of Physiology Biochemistry and Pharmacology. ,vol. 99, pp. 111- 181 ,(1984) , 10.1007/BFB0027716
M. Boshart, L. Gissmann, H. Ikenberg, A. Kleinheinz, W. Scheurlen, H. zur Hausen, A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. The EMBO Journal. ,vol. 3, pp. 1151- 1157 ,(1984) , 10.1002/J.1460-2075.1984.TB01944.X
J Burns, A K Graham, C Frank, K A Fleming, M F Evans, J O McGee, Detection of low copy human papilloma virus DNA and mRNA in routine paraffin sections of cervix by non-isotopic in situ hybridisation. Journal of Clinical Pathology. ,vol. 40, pp. 858- 864 ,(1987) , 10.1136/JCP.40.8.858
M. Durst, L. Gissmann, H. Ikenberg, H. zur Hausen, A papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 80, pp. 3812- 3815 ,(1983) , 10.1073/PNAS.80.12.3812