Regulation of olfactomedin 4 by Porphyromonas gingivalis in a community context.

摘要: At mucosal barriers, the virulence of microbial communities reflects outcome both dysbiotic and eubiotic interactions with host, commensal species mitigating or potentiating action pathogens. We examined epithelial responses to oral pathogen Porphyromonas gingivalis as a monoinfection in association community partner, Streptococcus gordonii. RNA-Seq cells showed that Notch signaling pathway, including downstream effector olfactomedin 4 (OLFM4), was differentially regulated by P. alone; however, regulation overridden S. OLFM4 required for cell migratory, proliferative inflammatory gingivalis. Activation induced through increased expression Notch1 receptor Jagged1 (Jag1) agonist. In addition, Jag1 released response gingivalis, leading paracrine activation. Following Jag1-Notch1 engagement, extracellular domain cleaved gingipain proteases. Antagonism gordonii involved inhibition activity secreted hydrogen peroxide. The results establish novel mechanism which modulates function is dependent on context. These interrelationships have relevance innate fate decisions health disease.