The secretome of human dental pulp stem cells protects myoblasts from hypoxia‑induced injury via the Wnt/β‑catenin pathway

摘要: Human dental pulp stem cells (hDPSCs) present several advantages, including their ability to be non‑invasively harvested without ethical concern. The secretome of hDPSCs can promote the functional recovery various tissue injuries. However, protective effects on hypoxia‑induced skeletal muscle injury remain explored. study demonstrated that C2C12 myoblast coculture with attenuated CoCl2‑induced hypoxic compared alone. hDPSC increased cell viability and differentiation decreased G2/M cycle arrest under conditions. These results were further verified using hDPSC‑conditioned medium (hDPSC‑CM). data revealed hDPSC‑CM depend concentration ratio CM. In terms underlying molecular mechanism, activated Wnt/β‑catenin pathway, which protein levels Wnt1, phosphorylated‑glycogen synthase kinase‑3β β‑catenin mRNA Wnt target genes. By contrast, an inhibitor (XAV939) diminished hDPSC‑CM. Taken together, findings alleviated potentially through regulating pathway. may provide new insight into a therapeutic alternative in hypoxia‑related diseases.