Imaging the bio-distribution of fluorescent probes using multispectral epi-illumination cryoslicing imaging.
作者： Athanasios Sarantopoulos , George Themelis , Vasilis Ntziachristos
DOI: 10.1007/S11307-010-0416-8
关键词: Fluorescence 、 Preclinical imaging 、 In vivo 、 Ex vivo 、 Multispectral image 、 Modality (human–computer interaction) 、 Optical imaging 、 Materials science 、 Epi illumination 、 Biomedical engineering
摘要: The increasing availability of fluorescent probes for in vivo optical imaging enables the interrogation complex biological processes small animals serving as models human-like tissue function and disease. However, validation probe bio-distribution during their development or study different disease models, support studies, is not straightforward. system developed consists a customized multispectral planar imager that has been adapted on commercial cryomicrotome provides powerful modality ex animals. ability to capture 3D anatomical (color) fluorescence volumetric distributions multiple markers high resolution showcased. Serving both method accurately agents inside organisms non-invasive methods, cryoslicing offers useful insights molecular probes.
springer.com
sci-hub.se 参考文章(37)
David P. Eisenberg, Prasad S. Adusumilli, Karen J. Hendershott, Sun Chung, Zhenkun Yu, Mei-Ki Chan, Michael Hezel, Richard J. Wong, Yuman Fong, Real-Time Intraoperative Detection of Breast Cancer Axillary Lymph Node Metastases Using a Green Fluorescent Protein-Expressing Herpes Virus Annals of Surgery. ,vol. 243, pp. 824- 832 ,(2006) , 10.1097/01.SLA.0000219738.56896.C0
Eiichi Tanaka, Hak Soo Choi, Hirofumi Fujii, Moungi G. Bawendi, John V. Frangioni, Image-Guided Oncologic Surgery Using Invisible Light: Completed Pre-Clinical Development for Sentinel Lymph Node Mapping Annals of Surgical Oncology. ,vol. 13, pp. 1671- 1681 ,(2006) , 10.1245/S10434-006-9194-6
Elizabeth M. C. Hillman, Anna Moore, All-optical anatomical co-registration for molecular imaging of small animals using dynamic contrast. Nature Photonics. ,vol. 1, pp. 526- 530 ,(2007) , 10.1038/NPHOTON.2007.146
Ralph Weissleder, Mikael J. Pittet, Imaging in the era of molecular oncology Nature. ,vol. 452, pp. 580- 589 ,(2008) , 10.1038/NATURE06917
Wolfgang Johann Weninger, Timothy Mohun, Phenotyping transgenic embryos: a rapid 3-D screening method based on episcopic fluorescence image capturing. Nature Genetics. ,vol. 30, pp. 59- 65 ,(2002) , 10.1038/NG785
James Sharpe, Ulf Ahlgren, Paul Perry, Bill Hill, Allyson Ross, Jacob Hecksher-Sørensen, Richard Baldock, Duncan Davidson, Optical Projection Tomography as a Tool for 3D Microscopy and Gene Expression Studies Science. ,vol. 296, pp. 541- 545 ,(2002) , 10.1126/SCIENCE.1068206
Meng-Lin Li, Jung-Taek Oh, Xueyi Xie, Geng Ku, Wei Wang, Chun Li, G. Lungu, G. Stoica, L.V. Wang, Simultaneous Molecular and Hypoxia Imaging of Brain Tumors In Vivo Using Spectroscopic Photoacoustic Tomography Proceedings of the IEEE. ,vol. 96, pp. 481- 489 ,(2008) , 10.1109/JPROC.2007.913515
Sylvie Kossodo, Maureen Pickarski, Shu-An Lin, Alexa Gleason, Renee Gaspar, Chiara Buono, Guojie Ho, Agnieszka Blusztajn, Garry Cuneo, Jun Zhang, Jayme Jensen, Richard Hargreaves, Paul Coleman, George Hartman, Milind Rajopadhye, Le Thi Duong, Cyrille Sur, Wael Yared, Jeffrey Peterson, Bohumil Bednar, Dual In Vivo Quantification of Integrin-targeted and Protease-activated Agents in Cancer Using Fluorescence Molecular Tomography (FMT) Molecular Imaging and Biology. ,vol. 12, pp. 488- 499 ,(2010) , 10.1007/S11307-009-0279-Z
A. Bogaards, H.J.C.M Sterenborg, J. Trachtenberg, B.C. Wilson, L. Lilge, In vivo quantification of fluorescent molecular markers in real-time by ratio imaging for diagnostic screening and image-guided surgery. Lasers in Surgery and Medicine. ,vol. 39, pp. 605- 613 ,(2007) , 10.1002/LSM.20525
David Wilson, Debashish Roy, Grant Steyer, Madhusudhana Gargesha, Meredith Stone, Eliot McKinley, Whole Mouse Cryo-Imaging Medical Imaging 2008: Physiology, Function, and Structure from Medical Images. ,vol. 6916, ,(2008) , 10.1117/12.772840