A cell cycle phosphoproteome of the yeast centrosome.
作者： J. M. Keck , M. H. Jones , C. C. L. Wong , J. Binkley , D. Chen
关键词: Protein phosphorylation 、 Cell biology 、 Centrosome cycle 、 Phosphorylation 、 Mitosis 、 Centrosome 、 Spindle apparatus 、 Spindle elongation 、 Cyclin-dependent kinase 、 Biology
摘要: Centrosomes organize the bipolar mitotic spindle, and centrosomal defects cause chromosome instability. Protein phosphorylation modulates centrosome function, we provide a comprehensive map of on intact yeast centrosomes (18 proteins). Mass spectrometry was used to identify 297 sites from different cell cycle stages. We observed modes phosphoregulation via specific protein kinases, site clustering, conserved phosphorylated residues. Mutating all eight cyclin-dependent kinase (Cdk)–directed within core component, Spc42, resulted in lethality reduced assembly. Alternatively, mutation one Cdk γ-tubulin (Tub4-S360D) caused delay aberrant anaphase spindle elongation. Our work establishes extent complexity this prominent posttranslational modification biology provides examples control function.
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